High-Throughput Screening for Induced Point Mutations
نویسندگان
چکیده
منابع مشابه
Scientific Correspondence High-Throughput Screening for Induced Point Mutations
With the completion of genome sequencing projects, emphasis in genomics has shifted from analyzing sequences to understanding gene function, and effective reverse genetic strategies are increasingly in demand. Here we report adaptations of the targeting induced local lesions in genomes (TILLING) reverse genetic strategy (McCallum et al., 2000a) to make it suitable for large-scale screening of c...
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TILLING (Targeting Induced Local Lesions in Genomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2001
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.126.2.480